ORIGINAL_ARTICLE
IMMUNOHISTOCHEMICAL EXPRESSION OF CD10 AND HER-2/NEU IN UROTHELIAL CARCINOMA VERSUS CHRONIC CYSTITIS IN ZAGAZIG UNIVERSITY HOSPITALS(RETROSPECTIVE STUDY)
Background: Urinary bladder tumor is the seventh most common worldwide tumor . According to National Cancer Institute in Egypt , urinary bladder tumor constitutes 30% of all cancer cases with an incidence rate of 13.5/100,000 individuals. CD10 protein has been an immediate part in pathways of transduction that control cellular development , apoptosis , metastatic possibilities of cancer cells and influence on invasion .CD10 has prognostic incentive in bladder cancer. HER2/neu protein seems to assume a part in neoplastic cell and included in pathogenesis of urinary bladder carcinoma. Association of HER2 receptor in proliferation of invasive urothelial carcinomas has incited trials to HER2/neu targeted therapies in advanced diseases.Aim of work: To evaluate the immunohistochemical expression of CD10 and HER2/ neu with the pathological parameters of urothelial carcinoma of urinary bladder well as grade and stage versus chronic cystitisMethods: CD10 and HER2/neu expressions were retrospectively analysed by immunohistochemistry in 30 cases of chronic cystitis and 30 paraffin-embedded specimens of patients with primary urothelial carcinoma (UC).The expressions were related to clinicopathological parameters ,well as grade and pathological stageResults: The immunohistochemial expression of CD10 and HER2/neu were negative(score 0) in chronic cystitis cases than urothelial carcinoma and was significantly (p < 0.05). Nineteen cases (63%) of primary urothelial carcinomas were positive for CD10 expression and 17 cases (56.7%) were positive for HER2/neu compared to chronic cystitis . The high grade of urothelial carcinoma and the high pathological stage were significantly connected with the high CD10 and HER2/neu expression (p < 0.05).Conclusion : High CD10 and HER2/neu expression was related to high grade and pathological stage of urothelial carcinoma.
https://zumj.journals.ekb.eg/article_4683_8b20e1108a85618d7b8c01630da4c916.pdf
2017-03-01
1
8
10.21608/zumj.2017.4683
CD10 – HER2/neu – urothelial carcinoma of urinary bladder – Immunohistochemistry (IHC)
Asmaa
Mohamed
1
Department of Pathology, Faculty of Medicine, Zagazig University, Egypt
AUTHOR
Mohamed
Ramadan
2
Department of Pathology, Faculty of Medicine, Zagazig University, Egypt
AUTHOR
Osama
Helmy
3
Department of Pathology, Faculty of Medicine, Zagazig University, Egypt
AUTHOR
Samah
Mohamed
4
Department of Pathology, Faculty of Medicine, Zagazig University, Egypt
AUTHOR
ORIGINAL_ARTICLE
ASSESSMENT OF RISK FACTORS FOR PRIMARY POSTPARTUM HEMORRHAGE AT ZAGAZIG UNIVERSITY HOSPITALS
Background: Primary postpartum hemorrhage PPH is a major cause of maternal morbidity and mortality. Many risk factors for primary PPH have been evaluated, while with changes of obstetric population and technological advances, some of these factors became more important and others were less. Aim: To identify common risk factors of primary PPH and measure the association between these factors and the occurrence of primary PPH. Methods: A case control study was conducted in obstetrics and gynecology department at Zagazig University Hospitals. Participants were selected by systematic random sampling. Data were collected about the participants’ age and social class as well as studied risk factors for primary PPH including previous delivery mode, previous antipartum hemorrhage APH, previous PPH, parity, birth weight, gestational age, maternal obesity, maternal hemoglobin level, labor induction, current delivery mode, delivery trauma, episiotomy, placenta previa and retained placenta. Results: Primary PPH was significantly higher among older ages (> 34 years). Primary PPH showed significant associations with the following factors; Previous APH (OR= 3.57), previous PPH (OR= 2.85), macrosomia (OR= 9.57), maternal anemia (OR= 5.37), obesity (OR= 4.01), vaginal delivery (OR= 2.13), labor induction (OR= 2.88), trauma (OR= 3.53), retained placenta (OR= 8.51), and placenta previa (OR= 3.51). Conclusion: Maternal anemia, retained placenta, Macrosomia, and previous PPH were the most significant risk factors for primary PPH. Recommendations: Organized antenatal visits are recommended to help early detection of PPH risk factors and improve females’ knowledge about PPH risk. clinicians should optimize care for high risk women. Adequate supplies should be provided to health facilities for prompt management of PPH.Running header: Risk Factors for Primary Postpartum Hemorrhage
https://zumj.journals.ekb.eg/article_4684_d6ad9ee11028b465682da5f47035db95.pdf
2017-03-01
1
9
10.21608/zumj.2017.4684
Postpartum Hemorrhage
risk factors
Pregnant
Zagazig
Amal
El Badawy
1
Public Health and Community Medicine Department, Faculty of Medicine, Zagazig University, Zagazig, Sharkia, Egypt
AUTHOR
Eman
Waly
2
Public Health and Community Medicine Department, Faculty of Medicine, Zagazig University, Zagazig, Sharkia, Egypt
AUTHOR
Nahla
Zaitoun
3
Public Health and Community Medicine Department, Faculty of Medicine, Zagazig University, Zagazig, Sharkia, Egypt
AUTHOR
Youssef
Abo-Elwan
4
Obstetrics and Gynecology Department, Faculty of Medicine, Zagazig University, Zagazig, Sharkia, Egypt
AUTHOR
ORIGINAL_ARTICLE
EXPRESSION OF LGR5 AND BETA-CATENIN IN BENIGN AND MALIGNANT COLORECTAL LESIONS IN ZAGAZIG UNIVERSITY HOSPITAL.
Background: Lgr5 -(GPR49) a Wnt target gene- is one of the most important cancer stem cell markers that have been isolated in colorectal cancer (CRC). The potentiation of Wnt/Beta-catenin signaling is now believed to mediate the self-renewal and proliferation of Lgr5 stem cells. Aim: to evaluate the expression of Lgr5 and Beta-catenin in colorectal cancer and benign colorectal lesions.Methods: Representative sections from thirty benign lesions [22 colorectal adenomas, 8 ulcerative colitis (UC)], and 30 CRC cases were stained by immunohistochemical technique using anti-Lgr5 and anti-Beta-catenin.Results: High Lgr5 expression was found in 0%, 60%, and 70% of normal mucosa, benign lesions, and CRC, respectively. Higher grades of Beta-catenin expression compared to normal expression was found in benign lesions as 23.3% for grade II and 6.7% for grade III and in CRC as 73.3% for grade II and 20% for grade III. Significant statistical differences between control groups, benign and malignant lesions in both Lgr5 and Beta-catenin expression were found. Conclusion: Lgr5 is highly expressed in CRC. That may suggest a role in carcinogenesis based on the CSC hypothesis. Also, Beta-catenin is activated in most CRC.
https://zumj.journals.ekb.eg/article_4685_27439830831ecae53a3945d73b627d7f.pdf
2017-03-01
1
10
10.21608/zumj.2017.4685
Cancer stem cell
Lgr5
Beta-catenin
CRC
Nelly
Said
1
Pathology ,Faculty of Medicine, Zagazig University, Zagazig 44519, Egypt,
AUTHOR
Amal
Mangoud
2
Pathology , Faculty of Medicine, Zagazig University, Zagazig,44519, Egypt,
AUTHOR
Salah
Mostafa
3
Pathology,Faculty of Medicine, Zagazig University, Zagazig,44519, Egypt,
AUTHOR
Mohamed
Yehia
4
General surgery departments,Faculty of Medicine, Zagazig University, Zagazig 44519, Egypt,
AUTHOR
Hesham
Abd El-Aziz
5
Pathology ,Faculty of Medicine, Zagazig University, Zagazig,44519, Egypt,
AUTHOR
ORIGINAL_ARTICLE
PROTECTIVE EFFECTS OF IVABRADINE (ALONE AND COMBINED WITH ATENOLOL OR ENALAPRIL) ON EXPERIMENTALLY-INDUCED ACUTE MYOCARDIAL INFARCTION IN ALBINO RATS
ackground: Cardiovascular disease (CVD) is the leading cause of death worldwide. Deaths are mostly attributable to coronary artery disease (CAD). Ivabradine, a pure heart rate lowering agent, acts by inhibiting the pacemaker If current in the sinoatrial node. It can be used for treatment of heart failure and stable angina with the main drugs for CVD.Objectives: To determine the cardioprotective effects of ivabradine pretreatment alone and in combination with each of atenolol and enalapril on acute myocardial infarction (MI) in rats.Materials & Methods: Eighty four rats were divided into seven groups (12 rats each): Group 1: sham-operated group; Group 2: control (untreated) MI group; Group 3: ivabradine-pretreated group; Group 4: atenolol-pretreated group; Group 5: enalapril-pretreated group; Group 6: ivabradine and atenolol-pretreated group; and Group 7: ivabradine and enalapril-pretreated group. Rats received drugs orally by gavage daily for 6 days. On the sixth day, acute MI was induced by persistent complete left coronary artery ligation. The parameters studied were mean arterial blood pressure, ECG changes (heart rate, ST height and T-wave voltage), serum levels of creatine kinase MB isoenzyme (CK-MB), cardiac troponin-I (cTnI), high-sensitivity C-reactive protein (hs-CRP) and N-terminal pro-brain-type natriuretic peptide (NT-proBNP), tissue levels of superoxide dismutase (SOD), reduced glutathione (GSH), Bcl-2-associated X protein (Bax) and the % of infarct size of the left ventricle.Results: Coronary artery ligation resulted in significant elevation in ST height, T-wave voltage, serum levels of CK-MB, cTnI, hs-CRP and NT-proBNP and tissue levels of Bax, with significant reduction of the tissue levels of SOD & GSH and produced 54% infarct size of the left ventricle. Oral pretreatment with ivabradine has cardioprotective effect against acute MI as evidenced by significantly lower ST height, T-wave voltage, serum levels of CK-MB, cTnI, hs-CRP and NT-proBNP and tissue levels of Bax, with significantly higher tissue levels of SOD & GSH and significantly lower infarct size (into 39%). Oral pretreatment with combination of ivabradine and atenolol produced greater cardioprotection against acute MI than observed with ivabradine alone evidenced by significantly lower ST height, T-wave voltage, serum levels of CK-MB, cTnI, hs-CRP and NT-proBNP and tissue level of Bax, with significantly higher tissue levels of SOD & GSH and significantly lower infarct size (into 23%). Although, the effects of oral pretreatment with combination of ivabradine and enalapril on ST height, T-wave voltage, tissue levels of SOD & GSH and % of infarct size were not significantly different from that of ivabradine; however, this combination caused significant reduction of serum levels of CK-MB, cTnI, hs-CRP and NT-proBNP and tissue level of Bax as compared to the effect of ivabradine alone.Conclusion: Ivabradine possesses protective effects against acute MI and these effects are lower than the protective effects of atenolol; but higher than that of enalapril. Concomitant administration of ivabradine with either atenolol or enalapril provides greater cardioprotection against acute MI than ivabradine alone and these effects are greatest when ivabradine is combined with atenolol.
https://zumj.journals.ekb.eg/article_4686_0334cd9d257950bf706c9037d0d0d355.pdf
2017-03-01
1
15
10.21608/zumj.2017.4686
Cardiovascular disease
Acute Coronary Syndrome
myocardial infarction
If current
Ivabradine
atenolol
Enalapril
Shireen
Mahmoud
1
Clinical Pharmacology Departments; Faculty of Medicine; Zagazig University
AUTHOR
Mohamed
Shehata
2
Clinical Pharmacology Departments; Faculty of Medicine; Zagazig University
AUTHOR
Ali
Moustafa
3
Clinical Pharmacology Departments; Faculty of Medicine; Zagazig University
AUTHOR
Gehane
El-Gendy
4
Clinical Pharmacology Departments; Faculty of Medicine; Zagazig University
AUTHOR
ORIGINAL_ARTICLE
CAUSES OF HEARING AID REJECTION IN GERIATRIC POPULATION OF EGYPTIAN RURAL AREAS
Background: Hearing loss affects a considerable proportion of geriatrics in Egypt. Although hearing aid technology is improving, there is still a high prevalence of hearing aid rejection in elder population. Causes of hearing aid rejection in rural geriatric population were not investigated in previous researches. Objective: This study aimed to detect causes of hearing aid rejection in rural geriatric population. Patients and Methods: Thirty rural elders of both genders, ≥ 65 years old, with ≥ moderately severe hearing loss participated in this study. They were subjected to history taking, Hearing Handicap Inventory for the elderly, queries about causes of HA rejection in rural areas, and basic audiological evaluation. Results: Psychosocial causes of hearing aid rejection were the more prevalent (36.7%), followed by audiological (20%), cultural believes (16.7%), device, and organic causes. Factors such as age, education, self-perceived disability, type and degree of hearing loss, and speech intelligibility affect the prevalence of theses causes. Conclusion: Identifying causes of hearing aid rejection and factors influencing them helps proper modification of hearing rehabilitation strategies.
https://zumj.journals.ekb.eg/article_4687_ca6404d5a82901a366b25c1afae12f5d.pdf
2017-03-01
1
10
10.21608/zumj.2017.4687
geriatrics
hearing aid rejection
Hearing loss
rural areas. Abbreviations: HL: hearing loss
HA: hearing aid
HHIE-S: Hearing Handicap Inventory for the Elderly (screening version)
LSD: least significant difference
PTA: pure tone average
SD: standard deviation
SNHL: sensorineural hearing loss
SRTs: speech reception thresholds
SRSs: speech recognition scores
X2: Chi-square
Ola
Ibraheem
1
Audio-vestibular Medicine, Audio-vestibular Unit, Otorhinolaryngology Department, Faculty of Medicine, Zagazig University, Egypt
AUTHOR
ORIGINAL_ARTICLE
SERUM LEAD, COPPER AND ZINC IN CHILDREN WITH BRONCHIAL ASTHMA
Background: Bronchial asthma is chronic inflammatory of the lung airways resulting in episodic airflow obstruction. Lead exposure results in alteration of immune system function resulting in asthma. Zinc, copper are component of antioxidant system and alteration of their level affect increase risk of asthma.Objective:. Determine the serum level of lead, zinc, copper in children with bronchial asthma and study their relation to development of asthmaPatients and methods: The patient group included 38 full-term newborn infants diagnosed as having bronchial asthma who were selected from pediatric department Benha Teaching Hospital. The control group included 17 healthy child age and sex matched C-reactive protein (CRP) were measured by the ELISA technique (highly sensitive CRP). ESR was performed by the Wintrobe method, Serum lead was determined by atomic absorption method. Serum copper and zinc were determined by calorimetric method.Results: Serum copper and zinc were elevated in asthmatic children compared to controls with no relation to clinical manifestations or laboratory parameters .Serum lead showed no difference among patients and controls however it increased in severe cases compared moderate and mild cases and correlated positively with duration of admission.Conclusion:. : We determined serum copper, zinc and lead in children with bronchial asthma. We found elevated serum copper and zinc in asthmatic children compared to controls with no relation to clinical manifestations or laboratory parameters .Serum lead showed no difference among patients and controls, however it increased in severe cases compared moderate and mild cases and correlated positively with duration of admission. These results indicate importance of environmental exposure as a risk factor of asthma in children
https://zumj.journals.ekb.eg/article_4688_fff552e49136bab13ef54a5eb0bf9f02.pdf
2017-03-01
1
11
10.21608/zumj.2017.4688
bronchial asthma
children
Copper Zinc
Lead
Wagdy
Elsayed
1
Department of Pediatric and Clinical Pathology, Benha teaching hospital
AUTHOR
Shahenaz
Alkalyouby
2
Department of Pediatric and Clinical Pathology, Benha teaching hospital
AUTHOR
Eman
Essa
3
Department of Pediatric and Clinical Pathology, Benha teaching hospital
AUTHOR