Zagazig University, Faculty of Medicine
Zagazig University Medical Journal
1110-1431
2357-0717
22
6
2016
11
01
EFFECT OF NESFATIN-1 ON ISCHAEMIA-REPERFUSION INJURY IN ISOLATED HEART OF ALBINO RATS
1
11
EN
Mohamed
Tawfik
Physiology Department, Faculty of Medicine, Zagazig University
Dalia
Abd Al-Aleem
Physiology Department, Faculty of Medicine, Zagazig University
Reham
Ibrahim
Physiology Department, Faculty of Medicine, Zagazig University
Rehab
Abaza
Physiology Department, Faculty of Medicine, Zagazig University
10.21608/zumj.2016.4671
Background: Nesfatin-1 is a hypothalamic neuropeptide which is involved in control of food intake and glucose homeostasis. Lower plasma level of Nesfatin-1 in patients with acute myocardial infarction (AMI) was reported.<br />Objective: This study was designed to explore possible effect of Nesfatin-1 on ischemia-reperfusion injury in isolated heart of adult male albino rats and to explain the possible involved mechanisms, in a trial to clarify Nesfatin-1 expected cardioprotective effect.<br />Design: This study was carried out on eighteen adult male albino rats which were divided equally (n=6) into 3 groups: Group I (ischemia-reperfusion I/R group); hearts were stabilized then subjected to (I/R) protocol, Group II (Nesfatin-1 pre-conditioning group); Nesfatin-1 was infused for 20 minutes before hearts were subjected to ischemia and Group III (Nesfatin-1 post-conditioning group); Nesfatin-1 was infused for 20 minutes at the beginning of 120 minutes of reperfusion. Cardiac performance indicators as left ventricular pressure (LVP), +max (LV dp/dt), -max (LVdP/dt)+, in addition to heart rate were recorded. Lactate dehydrogenase (LDH), creatine kinase-MB (CK-MB), superoxide dismutase (SOD) and C-reactive protein (CRP) were measured in the collected perfusate and cardiac Malondialdehyde (MDA) was measured. Finally, Nitro blue tetrazolium stain was used to detect the necrotic tissue percentage to the whole left ventricular mass.<br />Results: In group III (post conditioning group), there was a significant increase of the studied cardiac parameters compared to group I (I/R). Nesfatin-1 significantly increased LVP, +max (dp/dt), -max (dp/dt) and HR in comparison with I/R group. This was associated with a significant decrease in LDH and CK-MB levels, a significant increase in SOD level and a significant decrease in MDA and CRP levels. Moreover, Nesfatin-1 caused a significant decrease in percentage of necrotic tissue to the whole left ventricular mass. Regarding group II, no significant changes were detected in all parameters except for significant increase in SOD level and significant decrease in CRP level.<br />Conclusion: Nesfatin-1 protects against ischemia/reperfusion injury in vitro through its antioxidant and anti-inflammatory properties by limiting the infarction area, only if given as a post conditioning factor after I/R. Those results open the way to include Nesfatin-1 among the strategies for management of cardiac infarction during reperfusion.
Nesfatin-1,cardiac ischemia-reperfusion,Oxidative Stress,Inflammation
https://zumj.journals.ekb.eg/article_4671.html
https://zumj.journals.ekb.eg/article_4671_dc3f3b3f8d2d3bf8ab2455d54e17bbbb.pdf
Zagazig University, Faculty of Medicine
Zagazig University Medical Journal
1110-1431
2357-0717
22
6
2016
11
01
PROGNOSTIC VALUE OF NEURON SPECIFIC ENOLASE IN SERUM FOR OUTCOME OF INFANTS WITH BIRTH ASPHYXIA
1
7
EN
Hala
Attia
Depatments of Pediatrics ,Faculty of Medicine,Zagazig university
Mohammad
Holayl
Depatments of Pediatrics ,Faculty of Medicine,Zagazig university
Abd El-Razik
El-Sheikh
Depatments of Pediatrics ,Faculty of Medicine,Zagazig university
Yousri
Abo-Elmagde
Depatments of Medical biochemistry Faculty of Medicine,Zagazig university
10.21608/zumj.2016.4672
Background: Birth asphyxia [BA] and the resultant hypoxic ischemic encephalopathy [HIE] is a common cause of neonatal morbidity and mortality and neurologic disabilities among survivors .It is important to find an early and reliable indicator of brain damage and of poor long-term prognosis to initiate or end neuroprotective treatment.<br />Objective: Assess if neuron specific enolase [NSE] can be used as a serum biochemical marker of brain damage in neonates exposed to perinatal asphyxia [PA], and if it can be used in predicting long- term outcome in these infants.<br />Study design: This prospective study was carried out on 50 neonates who were delivered in the Obstetric Department of Zagazig University Hospitals and admitted to the neonatal intensive care unit [NICU]. The patient group comprised 30 full term neonates who developed symptoms and signs of HIE. Twenty healthy full term neonates of matched age, sex and weight served as a control group. All neonates were subjected to; history-taking, clinical examination and laboratory investigations, including measurement of serum NSE by enzyme immuometric assay. In addition survivor neonates were subjected to follow-up at 6 month and 12 month.<br />Results: Apgar scores and arterial cord blood pH were significantly lower in HIE neonates than that in control neonates. Meanwhile, serum NSE and arterial cord blood base deficit were significantly higher in HIE neonates than that in control neonates. Serum NSE was significantly higher in neonates suffering HIE grade II & grade III than that in HIE grade I& control neonates. Furthermore, serum NSE was very highly significantly increased in neonates who had neurological sequalae than that in control neonates.<br />Conclusion and recommendation: Serum NSE correlated significantly with HIE grades II&III and neonates who suffered neurological sequalae. So it may be used as a marker of grades II& III and neurological sequalae in asphyxiated neonates. However, it can not be used to prevent BA.
missed
https://zumj.journals.ekb.eg/article_4672.html
https://zumj.journals.ekb.eg/article_4672_74cbc74275cbaf330edf738ea444ccc6.pdf
Zagazig University, Faculty of Medicine
Zagazig University Medical Journal
1110-1431
2357-0717
22
6
2016
11
01
DWI-MR AND ADC VALUE IN CHARACTERIZATION OF DIFFERENT PANCREATIC MASSES"
1
10
EN
Esam
Hemat
Radiology department, faculty of medicine, Zagazig University
Ahmed
Ismail
Radiology department, faculty of medicine, Zagazig University
10.21608/zumj.2016.4673
Purpose: Our work was aiming to assess DWI and ADC values in characterization of different pancreatic masses.<br />Patients & methods; This cross sectional study carried out on 20 patients (9 males & 11 females), with a mean age of 48 years (ranged from 35 years to 67 years), from December 2013 till October 2015. All the patients were referred from general surgery, internal medicine, oncology departments and outpatient clinics of Zagazig University hospitals to radiology department for conventional MRI, DWI & ADC mapping. All patients gave a written consent before scanning.<br />.Results: The mean ADC value of benign pancreatic masses was 2.49x10-3 mm2/sec (at b= 1000 mm2/sec) and 3.006 x10-3 mm2/sec.(at b= 500 mm2/sec). The malignant pancreatic masses mean ADC value was 1.24x10-3 mm2/sec (b= 1000 mm2/sec) and was 1.34x10-3 mm2/sec (b= 500 mm2/sec). So, there is a significant difference between benign and malignant pancreatic masses and the mean ADC values of benign and malignant lesions done by using b=1000 are statistically higher than done by b=500. Sensitivity, specificity, PPV and NPV were 92.8%, 83.33%, 92.8%, 83.33% respectively at b value 500 mm2/sec. However, sensitivity, specificity, PPV, NPV were 100%, 83.33%, 93.33%, 100%, respectively at b value 1000 mm2/sec.<br />By using ADC cutoff value of 1.5x10-3 mm2/sec in differentiating benign and malignant pancreatic masses, the p value was < 0.001 at b value 1000 mm2/sec.<br />Conclusion: DWI has the advantage that it can be obtained without breath-holding, using respiratory triggered technique and it is simple and non-invasive. So, we recommend adding of DWI and ADC value in conjunction to conventional MRI as a useful modality in assessment and characterization of pancreatic masses, specially at b value 1000 s/mm2 to get a highest sensitivity and specificity.
Pancreatic carcinoma,pseudocysts,MRI,DWI and ADC values
https://zumj.journals.ekb.eg/article_4673.html
https://zumj.journals.ekb.eg/article_4673_ed1487b83150d81672bd113a9c106b9f.pdf
Zagazig University, Faculty of Medicine
Zagazig University Medical Journal
1110-1431
2357-0717
22
6
2016
11
01
EVALUATION OF THE ROLE OF HEPCIDIN IN PREDICTING THE THERAPEUTIC EFFICACY OF ERYTHROPOIESIS-STIMULATING AGENT TREATMENT IN PATIENTS OF CHRONIC RENAL FAILURE
1
9
EN
Hoda
Ahmed
Departments of Clinical Pathology ,Benha Teaching Hospital;Benha, Egypt
Amal
El-Maghraby
Departments of General Medicine ,Benha Teaching Hospital;Benha, Egypt
10.21608/zumj.2016.4674
Background: Chronic kidney disease is associated with increase serum hepcidin level which contributes to severity of anemia and to resistance of erythropoiesis stimulating agents and dysregulation of iron hemostasis. Serum hepcidin correlates positively with ferritin in patients on hemodialysis. Subjects and Methods: fifty patients of end stage renal disease(ESRD) on regular hemodialysis and on erythropoietin therapy, twenty seven males, twenty three females and twenty subjects as control. Group matched for age, gender were clinically assessed and laboratory investigations were done in the form of serum urea, creatinine, iron profile (serum iron, ferritin, total iron binding capacity), serum erythropoietin, serum hepcidin, and hemoglobin. Results: There is significant negative correlation between hepcidin and hemoglobin, iron, total iron binding capacity and erythropoietin (P<0.01) and positive significant correlation between serum hepcidin and serum ferritin (P<0.05) in both male and female groups. Hepcidin and serum ferritin are higher in patients groups than control but erythropoietin is lower in patients groups than control with statistical significance (P<0.01).
hepcidin,erythropoietin,chronic renal failure
https://zumj.journals.ekb.eg/article_4674.html
https://zumj.journals.ekb.eg/article_4674_efd84a51a01a84ddc91aca75ce9c4790.pdf
Zagazig University, Faculty of Medicine
Zagazig University Medical Journal
1110-1431
2357-0717
22
6
2016
11
01
EFFECT OF OBESTATIN ON CARDIOVASCULAR SYSTEM IN TYPE II DIABETIC RAT MODEL
1
17
EN
Mohammed
Ibrahim
Physiology department, Faculty of Medicine, Zagazig University
Nawal
Gerges
Physiology department, Faculty of Medicine, Zagazig University
Nadine
Raafat
Physiology department, Faculty of Medicine, Zagazig University
Shaimaa
Hadhoud
Physiology department, Faculty of Medicine, Zagazig University
10.21608/zumj.2016.4675
Background: Obestatin (OB) is a novel anorexiogenic peptide produced in the stomach. It has been shown to regulate glucose and lipid metabolism. However, its potential role in cardiovascular control is still not clear and controversial.<br />Aim of the work: To evaluate the in vivo and in vitro effects of Obestatin on cardiovascular system in both normal and diabetic rats. Also, to identify the possible mechanism of its action.<br />Material and methods: 40 healthy male albino rats weighing 180-200 gm. were used and divided into two main groups: In vivo experiments subdivided into normal (n= 10 ) and Type II Diabetes induced groups (n=10) In which heart rate and blood pressure were recorded before and after OB (100 Mg/kg body weight) injection , In vitro experiments subdivided into normal (n= 10) and Type II Diabetes induced group ( n= 10) in which the effect of OB (1 nmol/l) alone and its effect in presence of β –blocker propranolol (30Mmol/l) and α blocker prazosin (3Mmol/l) on amplitude and frequency of cardiac contractility were studied ,also effects of OB alone (100 pmol/l) and in presence of LNAME(0.3Mmol/l) on contraction induced by phenylephrine (10 Mmol/L) of isolated thoracic aorta. Type II Diabetes induced by feeding rats for two weeks a high-fat diet (58% fat, 25% protein, 17% carbohydrates as percentage of total calories). On day 13, rats were given a single intraperitoneal injection of streptozotocin (25 mg/kg body).<br />Results: Obestatin injection showed no significant difference on blood pressure and heart rate in both normal and diabetic rats, However in vitro studies showed that OB has both positive inotropic and chronotropic effects on isolated heart of both normal and diabetic rats which were blocked by propranolol but not by prazocin. Moreover, OB produced significant vascular relaxation of isolated rat thoracic aorta of the normal group which was attenuated by L-NAME in normal rats. However, the relaxation effect of OB was much weaker in diabetic rats and was blocked by L-NAME.<br />Conclusion: Obestatin improves cardiac function and exerts vasodilator effects via nitric oxide (NO) release, so it may be decrease systemic vascular resistance in type II diabetes.
Obestatin,type II diabetic rats,Propranolol,prazocin,isolated thoracic aorta
https://zumj.journals.ekb.eg/article_4675.html
https://zumj.journals.ekb.eg/article_4675_26b0004f1db640b784b1087fd48094cc.pdf
Zagazig University, Faculty of Medicine
Zagazig University Medical Journal
1110-1431
2357-0717
22
6
2016
11
01
UPPER ARM BASILIC VEIN TRANSPOSITION FOR HEMODIALYSIS: WHAT IS THE OPTIMAL TECHNIQUE?
1
7
EN
Mohammed
Alhewy
Vascular surgery unit, faculty of medicine- AlAzhar university, Assiut
10.21608/zumj.2016.4676
Objective: The brachiobasilic arteriovenous fistula (BBAVF) can be formed in one or two stages. This study examined the failure rates and functional patencies of one-stage vs two-stage brachiobasilic transposition fistulas to compare the two surgical techniques.<br />Patients and methods: 50 BB fistulae (25 single and 25 two-stage) were created over a 3year period (2012-2015). Data including sex, age, dialysis and diabetic status was collected from the case notes. Patency and time to maturity data was collected prospectively from the dialysis unit.<br />Results: During the study interval, 100 brachiobasilic transpositions (50 one-stage and 50 two-stage) were performed in 100 patients. Patients undergoing the two-stage procedure tended to be older (mean 49 vs 53 years; P = 0.8). The two-stage BBAVFs had significantly better primary (88% vs 92%; P=0.58), and secondary functional (92% vs 96%; P=0.508.) patencies. Pre-HD fistula has better patency than post-HD fistula (100% vs 90%; p=0.015)<br />Conclusion: This study describes a series of BBAVFs and makes comparison between the one-stage and two-stage operations. Significantly improved overall functional patency is demonstrated for the two-stage operation.
Brachiobasilic,Fistula,Transposition
https://zumj.journals.ekb.eg/article_4676.html
https://zumj.journals.ekb.eg/article_4676_71be841fca58c069c2d8f567ebc42403.pdf