Association between serum microrna-21 gene expression , carcinoemberyonic antigen and clinicopathological character for colorectal cancer

Document Type : Original Article

Authors

1 medical biochemistry,medicine,zagazig

2 medical biochemistry department/faculty of medicine/ zagzazig university /zagazig / sharkia

3 General SurgeryDepartment, Faculty of Medicine, Zagazig University, Zagazig, Egypt

4 medical biochemistry department /faculty of medicine/zagazig university/zagazig /sharkia

Abstract

Background and objetives: MicroRNAs (miRNAs) are small, noncoding RNAs that are involved in carcinogenesis through postranscriptional gene regulatory activity. The current study aimed to measure serum miR-21 expression levels and to evaluate their association with the outcome of colorectal cancer (CRC) and correlate it with carcinoemberyonic antigen level.
Methods: Blood samples were collected from 48 CRC patients and 48 healthy controls. The expression levels of miR-21 were measured using a quantitative reverse transcription polymerase chain reaction (qRT-PCR).The results were analysed to assess the relationship between serum miR-21 levels and clinic pathological and prognostic factors.
Results: A univariate analysis revealed that high expression levels of serum miR-21 were associated with higher local recurrence , TNM staging, PT staging ,venous invasion ,liver metastasis and recurrane (p=0.004),positively correlated with CEA.
Conclusion: The expression level of serum miR-21 correlates with CEA, recurrence and some pathological parameters of CRC patients. Our results suggest that circulating serum miR-21 is a promising prognostic tumour marker, and they highlight the potential clinical utility of miR-21 expression as a diagnostic and prognostic marker for CRC prognosis.

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