Zagazig University, Faculty of MedicineZagazig University Medical Journal1110-143126120200101Contribution of Non Traditional Cardiovascular Risk Factors to the Cardiovascular Disease in Patient with Type 2 Diabetes166257310.21608/zumj.2019.13277.1233ENHuda AbdelrhmanAmmar Al TrabulsiLibya, Benghazi University, Faculty of Medicine, Cardiology DepartmentMohamed HossamEl-ShaerProfessor of Cardiology, Faculty of Medicine - Zagazig UniversityMohamed MostafaEl-DaydamonyProfessor of Cardiology, Faculty of Medicine - Zagazig UniversityAlaa SalamaEl-SayedLecturer of Cardiology, Faculty of Medicine - Zagazig UniversityJournal Article20190527Background: T2DM is a major risk factor for heart failure, peripheral arterial insufficiency and microvascular complications, affecting life quality and expectancy. Diabetes also increases coronary death rates conferring the patient a worst prognosis after having the first CHD event. The mortality rate in non-diabetic patients was less compared to diabetic patients who experienced CHD. Objectives: to study was the association of nontraditional risk factors with incidence of coronary heart disease in Patient with Type 2 Diabetes. Patients & Methods: Our study had been carried out in cardiology department, Zagazig University from April 2017 to December 2017. The study included 105 patients divided into 3 groups; they were young and middle aged subjects selected conveniently according to specified groups (normal control group, diabetic control group without history of coronary artery disease and ischemic heart disease group with history of coronary artery disease. History and physical examinations were recorded and laboratory tests were performed for all patients. Results: showed that there ws a statistical significant difference regarding Diastolic dysfunction, Ventricular hypertrophy, Uric acid, Serum hsCRP, Serum fibrinogen and Homocystein in T2DM patients group. Conclusions: Cardiovascular risk factors were higher in subjects with diabetes compared to subjects without diabetes, Non-traditional cardiovascular risk factors may be related independently to incident coronary heart disease in T2DM patients.Zagazig University, Faculty of MedicineZagazig University Medical Journal1110-143126120200101Correlation between Uric Acid, Albuminuria and Cardiovascular Outcome In Patients With Diabetes Mellitus7163134310.21608/zumj.2019.12142.1212ENUsamaRagabInternal Medicine Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt0000-0001-5411-1943Fayrouz OthmanSelimInternal Medicine Department, Faculty of Medicine, Zagazig University, Zagazig, EgyptAhmad IbrahimElagrodyInternal Medicine Department, Faculty of Medicine, Zagazig University, Zagazig, EgyptMohamed Hossam ElidnMouradClinical Pathology Department, Faculty of Medicine, Zagazig University, Zagagzig, EgyptMohamed MohamedSakrInternal Medicine Department, Faculty of Medicine, Zagazig University, Zagazig, EgyptJournal Article20190426Background: Many studies have shown an association between uric acid (UA) and cardiovascular (CV) outcome in general population and diabetes, also the association between albuminuria and cardiovascular outcome is well established. The link between serum UA levels and albuminuria with subsequent CV affection is not yet clear. For this reason, we aimed to study such association. Method: One hundred and eight individuals with type 2 diabetes which were divided into two groups each constitute of 54 individuals according to presence or absence of CV disease. Routine laboratory investigations together with serum UA and urinary albumin to creatinine ratio (uACR) was analyzed in the two groups they were then correlated to each other and to different study parameters and future CV event using Framingham risk score (FRS). Results: The present study proved that there is statistical significant difference as regard risk of CVD as calculated by FRS being higher in diabetic patients with high serum UA (SUA) levels compared to those with low uric acid levels; the same apply for those with high urinary ACR. Serum UA was found to be correlated with urinary albumin execretion in patients with diabetes with and without CVD (n=108, r=0.450, pZagazig University, Faculty of MedicineZagazig University Medical Journal1110-143126120200101Evaluation of primary immunodeficiency in patients with meningitis in fever hospitals17273152910.21608/zumj.2019.11144.1155ENMarwa ElsayedEldarawyPediatric department, Faculty of Medicine, Zagazig University, pediatric department, faqus general hospital Sharkia, Egypt.MervatHeshampediatric department, faculity of medicine, Zagazig university099776655460009Mohamed AbdelkaderAlmalkyPediatric Department, Faculty of Medicine, Zagazig University, egyptAhmed ElsadekFakhrMicrobiology and Immunology Department,
Faculty of Medicine, Zagazig University, Egypt0000-0002-0497-4908Journal Article20190407Background: The hallmark of primary immunodeficiency diseases is increased susceptibility to recurrent, severe and opportunistic infections. In this study we evaluate patients with meningitis for underlying primary immunodeficiencies.<br /><br />Methods: During the period from August 2016 to February 2017, thirty infant and children patients (16 male and 14 female) with age ranged from 40 days to 15 years participated in the study were admitted to Faqus and Zagazig fever hospitals with meningitis were evaluated for primary immunodeficiencies by measurement of serum immunoglobulin levels, assessment of T-Cell Receptor Excision Circles and assessment of complement system by CH50 assay.<br /><br />Results: This study showed that (50%) had deficient IgG, (20%) had deficient IgA, (30%) hyper IgE, (30%) hyper IgM and 10% Deficient in all Immunoglobulins. The study also showed that (43.3%) of the study group had TREC deficiency, (36.7%) were CH50 deficient and 26.7 had both TREC and CH50 deficiency. <br /><br />Conclusions: Meningitis may be the presenting manifestation of immunodeficiency diseases as there is high incidence of primary immune deficiency in patients with meningitis. TRECS and CH50 are decreased in most cases of meningitis which indicate high percentage of primary immunodeficiency diseases among those patients.Zagazig University, Faculty of MedicineZagazig University Medical Journal1110-143126120200101The Relation Between the Red Cell Distribution Width and Coronary Artery Calcium Scoring In Diabetic Patients Undergoing Coronary CT Angiography.28376668910.21608/zumj.2019.16165.1445ENMostafa MohamadAssyDepartment od Radiodiagnosis, Zagazig University, Zagazig, Egypt0000-0001-6221-6681Marwa FathyKhalilDepartment of Radiodiagnosis, Zagazig University, Zagazig, EgyptAMAlaa EldinDepartment of Radiodiagnosis, Faculty of medicine, Zagazig University, Egypt0000-0002-2271-6806Journal Article20190825Abstract<br />Background/Aim: This study was done to assess the relation between red cell distribution width (RDW) and coronary artery calcium score (CACS) in the diabetic patients undergoing coronary CT angiography and to assess the relation between RDW and severity of coronary artery disease. The objectives are to identify the role of red cell distribution width (RDW) in predicting coronary artery disease complexity and to evaluate the relationship between RDW and calcium score in diabetic patients.<br />Methods: Thirty diabetic patients were subjected to; history taking, complete physical examination, laboratory investigations including RDW (SD & CV) as a routine examination in complete blood count, erythrocyte sedimentation rate (ESR), lipid profile. Then multidetector CT was done for coronary artery examination and calcium scoring. <br /> Results: higher RDW (SD and CV) were associated with higher calcium score and the presence of greater coronary complexity of coronary artery disease. Cut off value of RDW to presence of coronary artery disease was 11.85% and RDW (SD) cut off was 39.5 fl, while cut off value of RDW (CV) to predict high calcium score (more than 100 ) was 12,95% and RDW (SD) more than 40.5 fl.<br /> Conclusions: a greater baseline RDW (CV and SD) value may be used as an indicator of CAD and high calcium score.Zagazig University, Faculty of MedicineZagazig University Medical Journal1110-143126120200101Description of Clinical and Microbiological Characteristics of Ventilator Associated Pneumonia Patients at Surgical Intensive Care Unit.38453394610.21608/zumj.2019.12056.1207ENMichael AdelShakeranaethesia and surgical intensive care, fuculty of medicine, zagazig universitySahar MohammadSaad-eldeenProfessor of Anesthesiology and surgical intensive care,
Faculty of Medicine, Zagazig University, EgyptHeba MahammadHelmyAssociate Professor of Anesthesiology and surgical intensive care,
Faculty of Medicine, Zagazig University, Egypt0000-0003-3539-4364Rehab HosnyEl-SokkaryMedical microbiology and immunology, faculty of medicine, Zagazig University, Zagazig, Egypt0000-0002-8135-7671Journal Article20190418Abstract:<br />Introduction: Ventilator-associated pneumonia (VAP) is considered one of the most widespread infections present in the intensive care units. It is associated with increase in hospitalization, ventilation days and mortality. The aim of this study is to determine the incidence, bacteriology, impact and clinical outcome of VAP patients.<br />Methods: Prospective observational non interventional study of VAP cohort, conducted in surgical intensive care unit (ICU), Zagazig University Hospitals over a period of 1 year (June 1, 2016–May 31, 2017). This study was approved by the Institutional Review Board, faculty of medicine, Zagazig University. Informed written consents were taken from first degree relatives.<br />Results: incidence of VAP was 9.94%. 76.5% of those patients were male and 23.5% were female. Gram negative bacteria were the main causative organisms in which klebsiella pneumonia was the predominating one. There was significant increase in ventilation days and length of stay (LOS) in ICU. APACHI II score was also significantly higher in VAP patients. VAP cases ware associated with higher mortality and lower cure rate. <br />Conclusion: VAP is a serious ICU acquired infection with significant impact and required effective preventive action.<br />Keywords: Ventilator-associated pneumonia, bacteriology, outcome.Zagazig University, Faculty of MedicineZagazig University Medical Journal1110-143126120200101The Role of Selenium Deficiency In Dilated Cardiomyopathy46523152710.21608/zumj.2019.12220.1211ENGhada MohammedAbdellatifpediatric department,faculty of medicine ,zagazig university,elsharkia,EgyptSaed MohammedMorsypediatric departement, faculty of medecine,zagazig university,Elsharkia,EgyptNahed MahmmoudKhaterdepartement of pediatric ,faculty of medicine ,zagazig university,elsharkia,EgyptHanan SamirAhmedclinical pathology,faculty of medecine,zagazig university,elshakia,EgyptJournal Article20190426ABSTRACT: <br /> BACKGROUND:<br /> It has been speculated that trace elements may play a role in the pathogenesis of dilated cardiomyopathy (DCM). In the present study, we aimed to assess serum concentrations of selenium (Se) in pediatric patients with idiopathic dilated cardiomyopathy (IDC) and to evaluate the correlation between serum Se concentrations and echocardiographic parameters.<br /> SUBJECTS AND METHODS: <br /> This study included 16 patients with IDC and 16 healthy controls. Serum level of selenium was measured by ELIZA method. Echocardiographic parameters including ejection fraction and fractional shortening were measured in all patients with IDC in order to evaluate its correlation with serum Se concentrations.<br /> RESULTS: <br />Serum concentration of Se in IDC patients was significantly lower than in healthy controls (pZagazig University, Faculty of MedicineZagazig University Medical Journal1110-143126120200101VITAMIN D LEVELS ASSESSMENT IN RELATION TO CORONARY MDCT ANGIOGRAPHIC FINDINGS53635515610.21608/zumj.2019.16328.1470ENHeba F.Tantawyradiology department, Faculty of medicine0000-0001-5932-303XAliHasaninRadiology department, Zagazig University0000-0001-8634-969xOmarFaridCardiology department, Zagazig University0000-0001-6913-2907Journal Article20190903ABSTRACT<br /><br />Introduction: Although vitamin D has a major role at the skeletal mineralization, it has been implicated at cardio vascular system, owing to abundance of its receptor there. Atherosclerosis is the most presenting symptom of such system that warrants the urge to detect the effect of vitamin D serum level changes in progression of the atherosclerosis. <br />Objectives: assess the correlation between vitamin D level and coronary artery findings using MDCT.<br />Patients and methods: 250 patients were included in our study, all of them underwent MDCT for coronary artery angiography, and then the findings were compared with vitamin D serum levels. <br />Results: 4 % of all participants (n=10) had vitamin D deficiency, 44 % (n=110) had insufficient levels of vitamin D, 52 % have sufficient vitamin D level (n=130). There is significant positive correlation between serum vitamin D levels and coronary artery calcium score (CACS) with PZagazig University, Faculty of MedicineZagazig University Medical Journal1110-143126120200101Plasma MicroRNA-133a as a Potential Predictor for Coronary Artery Stenosis Severity64743477510.21608/zumj.2019.13335.1238ENHeba Fawzy El SayedTurkyMedical Biochemistry & Molecular Biology Department, Faculty of Medicine, Zagazig University000-0002-9497-1662Wafaa Abdel LatifEmamMedical Biochemistry & Molecular Biology Department, Faculty of Medicine, Zagazig UniversitySally MShalabyMedical Biochemistry Department-Faculty of Medicine- Zagazig University-Egypt0000-0002-2868-020XNader TalaatKandilCadiology Department , Faculty of Medicine , Zagazig UniversityJournal Article20190530Background: Several scoring systems tried to determine coronary artery stenosis severity to investigate its correlation with laboratory parameters.<br />Aim of the study: To investigate the correlation between the expression levels of circulating miRNA-133a & coronary artery stenosis severity .<br />Patients and Methods: The study was conducted in Medical Biochemistry Department and Cardiology Department - Faculty of Medicine, Zagazig University& Medical Scientific Research Center. It included 72 subjects classified into 2 groups; Group (1): included 18 healthy subjects served as a control. Group (2 included 54 acute coronary syndrome patients. It was subdivided into 3 subgroups each 18 patient: unstable angina , non-ST-segment elevation myocardial infarction and ST-segment elevation myocardial infarction . Plasma miRNA-133a expression level was analyzed by real time PCR. Cardiac Cathetrization was done for showing the severity of stenosis. <br />Results: The present study showed high significant positive correlation between miRNA-133a level and coronary stenosis percentage in unstable angina group (pZagazig University, Faculty of MedicineZagazig University Medical Journal1110-143126120200101CHADS2 score as a Predictor of acute kidney injury in Diabetic Patient undergoing Elective Coronary intervention75863477610.21608/zumj.2019.12188.1213ENAhmed MohamedSaadcardiology department zagazg universityTarek SaidZolfakarcardiology department zagazg university1234-1234-1234-1234Nader TalatKandilcardiology department zagazg universityAlaa ElsayedSalamaCardiology Department , Faculty of medicine ,Zagazig University , Zagazig , EgyptJournal Article20190520Background: one of major adverse outcome of cardiac catheterization is acute kidney injury (AKI), and is associated with short-term and long-term mortality and morbidity .The pathogenesis of AKI is still not established although it is thought that the mechanism is medullary hypoxia lead to renal tubular dysfunction. <br />Objective: The aim of the study is to assess the efficacy of CHADS2 score in prediction of AKI in diabetic patients after percutaneous coronary intervention (PCI).<br />Methodology: This study was prospective cohort study done on 60 patients have diabetic history divided into two groups according CHADS2 score. <br />All diabetic patients underwent elective PCI.<br />All patients had the following: complete blood count, renal function, glycosylated hemoglobin (HbA1C), Resting electrocardiography, echocardiography Doppler study.<br />Serum creatinine was assessed before intervention, 48 hours after exposure to contrast media in PCI. Creatinine clearance was assessed also before and 48 hours after the intervention.<br />Results: AKI developed in eight patients ((13.3%) one patient with CHADS2 score < 3 and 7 patients have CHADS2 score >3.<br />The result showed that CHADS2 score is an independent predictor for incidence of AKI [odds ratio (OR) =8.111; 95% confidence interval (C.I) = 1.096 – 60.011; p=0.04].<br />There was a significant increase in AKI incidence with increased CHADS2 score<br />Conclusion: CHADS2 score is more accurate and sensitive in diagnosis of acute kidney injury after coronary intervention in comparison with old complicated scoring system.Zagazig University, Faculty of MedicineZagazig University Medical Journal1110-143126120200101Serum Interleukin-17 Level in Patients with Rheumatoid Arthritis and its Relation to Disease Activity87933134410.21608/zumj.2019.11577.1196ENShimaa EssamAtwaMedical Microbiology and Immunology Department, Faculty of Medicine - Zagazig UniversityMagda MostafaAzabProfessor of Medical Microbiology and Immunology, Faculty of Medicine - Zagazig UniversityMervat SolimanMohamedProfessor of Medical Microbiology and Immunology, Faculty of Medicine - Zagazig UniversityMazen MohamedEl SheikhProfessor of Rheumatology and Rehabilitation, Faculty of Medicine - Zagazig UniversityJournal Article20190409Background: Rheumatoid arthritis (RA) is a disease of progressive inflammatory autoimmune nature with articular and systemic effects. Its definite cause is unknown, but environmental and genetic factors are contributory. Aim of the Work: The aim of this study was to estimate the serum level of IL-17 in cases with RA and investigate its relation with disease activity among Egyptian population. Subjects and Methods: This study included 20 RA cases and 20 healthy control subjects. All cases were subjected to full history taking, thorough clinical examination, laboratory investigations including erythrocyte sedimentation rate (ESR), C-reactive protein (CRP), anti-cyclic-citrullinated peptide (anti-CCP) antibodies and rheumatoid factor (RF). Enzyme linked immunosorbent assay was used to measure serum IL-17 level. Disease activity score-28 (DAS-28) was assessed. Results: The mean disease duration of the cases was 5.95±2.52 years (2–10years). DAS-28 ranged between 2.4 and 6.1 with a mean of 3.87±1.32. Serum IL-17 level was higher in the RA group (226.6±215.6 pg/ml) than the control group (48.17±54.9pg/ml) with p-value of 98.2 pg/ml had sensitivity and specificity of 81.2% and 75%, respectively. Conclusion: Serum IL-17 level was significantly increased in cases with RA compared to healthy controls. Its significant correlation with DAS-28 suggested that serum IL-17 level could be an important marker of disease activity in RA.Zagazig University, Faculty of MedicineZagazig University Medical Journal1110-143126120200101The Role Of Alpha Diol G In Acne Vulgaris In Females94983394510.21608/zumj.2019.10980.1159ENAfaf HelmyGhanemDermatology & Andrology Department, Faculty of Medicine, Zagazig University, Egypt.Mohamed MahmoudNasrDermatology, Venereolody &amp; Andrology Dept
Faculty of Medicine
Zagazig University
EgyptHanaa HosnyElsayedClinical Pathology Department, Faculty of Medicine, Zagazig University, EgyptAminaMohamedDermatology & Andrology Department, Faculty of Medicine , Zagazig University, Egypt.Journal Article20190411Acne vulgaris in adult women is a chronic inflammatory disorder of pilosebaceous unit with many relapse cases. It commonly has psychological and social impact that interfere with the quality of life of many patients. Androgenic hormones have a very important role in the pathogenesis of acne. Androgens affect many functions in our body therefore, any alternation in androgen receptors may have an important implication in the development of the signs of hyperandrogenic states in women. The early diagnosis of these hyperandrogenic states is a very important point for the proper evaluation of the patient of acne and for the better choice of therapeutic management. Defining a pattern for laboratory profile analysis is very useful in the control of relapse cases of acne vulgaris in adult women. Which lately has been the aim of many of published studied.<br />Aim to establish the relation between 3 alpha-diol G levels and acne in female patients with normal androgenic status without menstrual dysfunctions in comparison with controls, in an attempt to elucidate its role in the pathogenesis of acne vulgaris which might help in treatment.Zagazig University, Faculty of MedicineZagazig University Medical Journal1110-143126120200101Prediction of Oesophageal Varices in Cirrhotic Patients by Serum- Ascites Albumin Gradient991076237910.21608/zumj.2019.16241.1465ENAhmedMarakbiTropical department, Faculty of medecine , Zagazig University, Egypt.Taghrid MohamedAbdallaTropical Department , Faculty of Medicine,Zagazig University ,EgyptSameh MahmoudAbdel MonemTropical department, Faculty of Medicine,Zagazig University . Egypt.Heba FouadPashaMedical Biochemistry Department , Faculty of Medicine,Zagazig University. Egypt.0000-0000-0984-4329Journal Article20190828Abstract<br />Background and aim of the work: Portal hypertension (PHT) and the eventual Oesophageal varices (OV) is one of the awful complications of liver cirrhosis. Bleeding from OV is a real leading cause of death. This study aims at evaluating serum ascites albumin gradient (SAAG) as a predictor of OV. Patients and methods: A descriptive cross-sectional study was conducted on 105 males and 26 females where they were divided into two main groups and sub groups according to the presence and size of OV. All participants were subjected to full history taking, thorough clinical examination, laboratory investigations, liver function tests, complete blood count, kidney function tests, hepatitis markers: HCV-Ab (anti hepatitis c virus antibody) hepatitis B surface antigen (HBs-Ag), abdominal ultrasonography and abdominal diagnostic paracentesis with calculation of SAAG. Results: there is highly significant increased SAAG in cirrhotic patients with OV than those without. Moreover, SAAG increases significantly with the grade of O.V. Conclusion: Serum ascites albumin gradient (SAAG) could be used as a non-invasive predictor for the presence of O.V in cirrhotic patients with ascites. Moreover, SAAG value increased significantly with the progression of O.V.Zagazig University, Faculty of MedicineZagazig University Medical Journal1110-143126120200101The sensitivity of optic nerve computed tomography scan vs optical coherence tomography in diagnosis of Idiopathic intracranial hypertension1081135952910.21608/zumj.2019.16891.1520ENAhmed MohammedAlaaRadiodiagnosis,faculty of Medicine,Zagazig university,Zagazig, Egypt0000-0002-2271-6806MohammedMahdyNeurology department,Faculty of Medicine ,Zagazig University ,Zagazig , Egypt0000-0002-8861-1687NesmaGhonemiNeurology department,Faculty of Medicine, Zagazig University , Zagazig ,EgyptJournal Article20190922Abstract<br />Background/Aim: This study aimed to evaluate the usage of optic nerve sheath diameter (ONSD) and Spectral-domain OCT for initial detection of elevated ICP in patients with IIH as an alternative to the invasive measurement of ICP. We also, try to find correlation between the findings of the two methods in those patients.<br />Methods: Forty patients presented with headache and fulfilled modified dandy criteria for IIH underwent post contrast multislice orbit computed tomography (CT) scan & spectral domain OCT (SD-OCT) scanning . Routine lab., and plain chest X-ray were done to exclude those patients with end organ failure. .<br /> Results: The estimated statistical cutoff value of ONSD was 5.5 mm with sensitivity of 84.4% and a specificity to diagnose optic nerve thickening by 100% in the left side and 85.7% in the right side .<br /> Conclusions: The addition of OCT to ONSD by post contrast multislice CT can increase its diagnostic ability for the cases with IIH, which may reduce the need for invasive diagnostic techniques like lumbar puncture .Zagazig University, Faculty of MedicineZagazig University Medical Journal1110-143126120200101Value of Anti-Müllerian Hormone in Diagnosis and Management of Children with Disorders of Sex Development1141223145710.21608/zumj.2019.11117.1163ENIbrahim ShawkyIbrahimDepartment of pediatrics, zagazig universityHossam MostafaKamalpediatrics department, faculty of medicine, zagazig universityAshgan Abd AllahAl-GobashyDepartment of Pediatrics, Faculty of Medicine, Zagazig University, EgyptEbtehag HelmyHassanDepartment of clinical pathology, Faculty of Medicine, Zagazig University, EgyptJournal Article20190330Aim: to investigate the serum levels of AMH in children with disorders of sex development and its correlation with other diagnostic investigations to assess the ability of AMH to predict the presence and function of testicular tissue.<br />Subjects& Methods: The study was carried out at the pediatric, pediatric surgery and clinical pathology departments at Zagazig University Hospitals (ZUH) during the period from February 2018 to December 2018. The study was performed on 40 infants and children below the age of ten years. They were divided into two groups, control and DSD groups, each group contained 20 participants. Children were subjected to full history taking, precise clinical examination, and investigations that included ultrasonography, karyotyping and biopsies. In addition, serum AMH, testosterone and dihydrotestosterone (DHT). Statistical analysis using suitable significance tests was performed.<br />Results: Specificity and sensitivity of testosterone and DHT in detection of testicular activity were 100% for both while they were 91.7% and 100% respectively for AMH in 46,XY DSD.<br />Conclusion: Serum testosterone and DHT had a better diagnostic role in 46.XY DSD than AMH while in 46,XX DSD, AMH is better.Zagazig University, Faculty of MedicineZagazig University Medical Journal1110-143126120200101Association between glucocorticosteroid receptor (NR3C1) gene polymorphism and bronchial asthma in children1231313145810.21608/zumj.2019.11975.1204ENKhalid MohamedSalahpediatric department, Faculty of Medicine, Zagazig University, Zagazig, EgyptMona MohamedAl Shafiepediatric department, Faculty of Medicine, Zagazig University, Zagazig, EgyptOsama AbdelAzizGaberMedical Biochemistry Department, Faculty of Medicine, Zagazig University, Zagazig, EgyptMahmoud TharwatAwadpediatric department, faculty of medicine, Zagazig University, Zagazig EgyptJournal Article20190415Abstract<br /><br />Introduction: There is a large variation in the magnitude of the response to asthma medications. Pharmacogenetics is responsible for a significant part of this variation.<br /><br />Objectives: we aimed at studying the effect of the Glucocoricoid receptors NR3C1 BCLI single nucleotide polymorphism (SNP) on the susceptibility to bronchial asthma in children and to evaluate its effect on the response to inhaled corticosteroids (ICS).<br /><br />Methodology: Seventy five asthmatic children and a control group of 66 non asthmatic children were included in the study. The level of asthma symptom control and pulmonary function tests were measured initially and 3 months after treatment with inhaled corticosteroids. The genotypes were studied using PCR-RFLP method.<br /><br />Results: No statistically significant difference was found between asthmatic group and the control group as regard the studied genotype. Among asthmatic children, The CC genotype was statistically associated with controlled asthma symptoms 3 months after treatment and the GG genotype was associated with poor asthma symptom control. Also, FEV1% after 3 months of treatment was statistically lower in children with the GG genotype as compared to children with the CG and CC genotypes<br /><br />Conclusion: glucocorticoid receptor NR3C1 SNP was not associated with asthma susceptibility in the studied group. However, the presence of the GG genotype was associated with decreased response to ICSs among asthmatic children as regards asthma symptom control and FEV1% response.Zagazig University, Faculty of MedicineZagazig University Medical Journal1110-143126120200101Interleukin 27 Serum Level And Its Prognostic Significance In Children With Immune Thrombocytopenia1321393603710.21608/zumj.2019.11145.1156ENTamer HasanHassanFaculty of Medicine, Zagazig University, Department of pediatrics, Sharkia EgyptAdel SherifAhmedFaculty of Medicine, Zagazig University, Department of pediatrics, Sharkia EgyptNermin RaafatAbdel-FattahMedical Biochemistry, Faculty of Medicine, Zagazig University, EgyptMarwa AliAhmedzagazig university faculty of medicine pediatric department,Sharkia EgyptJournal Article20190502Aim of the work: was to determine the level of IL-27 in patients with immune thrombocytopenia and its relationship to demographic,clinical and laboratory characteristics of patients as well as disease chronicity and response to treatment. Methods: A case control study carried out in Pediatric Hematology outpatient clinic , Zagazig University Hospitals. It included 80 subjects were divided into 4 group 1 included 20 patients with denovo ITP, group 2 included 20 patients with chronic ITP, group 3 included 20 patients with complete remission after 1st line therapy and group 4 (control group) included 20 healthy children as a control group. Results: there is significant difference regarding WBCs and Platelets, there was no significant difference in patients with ITP as regards hemoglobin level. ITP patients had significantly higher levels of IL-27 than controls. patients with acute ITP had the highest levels of IL-27 among patient groups ,while, patients in remission had the lowest IL-27 levels among patient groups. There was significant relationship between 1st line therapy and serum IL-27 in patients with ITP. Patients who received IVIG and combined steroids and IVIG had significantly higher IL-27 levels than others. There was significant relationship between 2nd line therapy and serum IL-27 in patients with chronic ITP. Where patients who received Eltrombopag had significantly lower IL-27 levels than others. Conclusion: we propose that the using of IL-27 as a predictor for ITP occurrence and for responsiveness to treatment but this need to be confirmed in larger studies.Zagazig University, Faculty of MedicineZagazig University Medical Journal1110-143126120200101Role of Oral Zinc Supplementation in Reduction of Neonatal Morbidity and Mortality in Zagazig University Hospitals1401476092610.21608/zumj.2019.16235.1454ENHebaGamalDepartment of Pediatric Faculty of Medicine &ndash; Zagazig University, EgyptSamarMahmoudDepartment of Pediatric Faculty of Medicine – Zagazig University, EgyptJournal Article20190912Background: Zinc is an essential cofactor for several hundred enzymes with a multitude of functions. Zinc is vitally important for proper immune functions, because of its role in skin and mucosal barrier function as well as humoral and cellular immunity. Zinc deficiency limits growth and development in infancy and childhood. Severe zinc deficiency may be observed in preterm infants especially if necrotizing enterocolitis coexisted. This study aimed to evaluate the ability of extra zinc doses to improve immunity, decrease disease incidence and death rate and show its effect on development and growth of preterm and term neonates. Methods: The present study was performed in the period from February 2016 to February 2017 in pediatrics department of Zagazig University Hospitals. 90 patients (45 patient in group I zinc supplementation, 45 patiant in group II without zinc) were enrolled in single blind randomized case control study Results: This study revealed that there was a considerable decrease in morbidity and death rates in correlation with the use of extra doses of zinc (10 mg/day for 14 day). Conclusions: Zinc supplementation can decrease the rate of morbidity and mortality, Zinc is vitally essential for proper immunityZagazig University, Faculty of MedicineZagazig University Medical Journal1110-143126120200101Frequency of Congenital Malformation in Neonatal Intensive Care Unit in Benghazi- Libya1481553783210.21608/zumj.2019.13417.1243ENSahar MohamedFarag SanfazLibya, Benghazi University, Faculty of Medicine, Pediatric DepartmentAlaa ZedanMohamedProfessor of pediatrics, Faculty of Medicine, Zagazig University, Department of pediatrics , Sharkia EgyptHany ElsayedMohamedAssistant Professor of pediatrics, Faculty of Medicine, Zagazig University, Department of pediatrics, Sharkia EgyptJournal Article20190602Background: Birth defects, encountered frequently by pediatricians, are important causes of childhood morbidity and mortality. Objectives: The work is to detect the frequency of the occurrence of congenital anomalies in neoborn admitted to NICU in Benghazi – Libya in the previous 5 years (2012-2016). Patients & Methods: Studied all neonate admitted during the study period from (2012-2016). Data were collected with review of records in all files of neonates born with congenital anomalies in Jumhoria hospital and Benghazi Medical Center along the duration between January 2012 and the end of 2016. Results: Demographic data for newborns studied showed that most cases with congenital malformations were male (66.2%) and females (33.8%) of all neonates with congenital malformations. Mothers ranged in age from 19 to 49 years. The average age of the mother was 35 years. Conclusions: Most frequent congenital malformation in newborn admitted to NICU in Benghazi – Libya in the previous 5 years (2012-2016) was congenital heart disease (CHD) followed by congenital neural tube defects. The year 2016 had the highest annual rate of congenital malformations.Zagazig University, Faculty of MedicineZagazig University Medical Journal1110-143126120200101EVALUATION OF NEOPTERIN AS A PROGNOSTIC MARKER FOR SEVERITY OF PNEUMONIA IN PEDIATRICS1561644141910.21608/zumj.2019.11318.1178ENMahmoud MohamedRabiePediatric,medicine,zagazigDina MohamedShokrypediatrc,medicine,zagazigYousif MohamedYousfpediatric,medicine,zagazigAmal FathyGhareebmedical biochemistry,medicine,zagazigJournal Article20190410Background: The aim of this study was to investigate the role of Neopterin in evaluation of severity and outcome of pneumonia in pediatric Department of Zagazig University Hospitals.<br />Methods: A prospective case control study was carried out at the pediatric Department of Zagazig University Children Hospitals during the period from November 2017 to December 2018 .sixty infants and children patients participated in the study 40 were diagnosed as pneumonia admitted to Pediatric Department of Zagazig University as case group And twintyof control group.<br />Results: In our study there was statistically significant difference between case and control groups in Neopterin level, there is statistically significant difference in Neopterin level between cases that need ICU dmission and M.V. and cases treated in ward only without need, there was statistically significant difference in Neopterin level between survivors and non-survivors. In this study, there was statistically significant difference in Neopterin level with disease severity.in which Neopterin is higher in severe than moderate and mild cases. Our study shows that there was statistically significant positive correlation between Neopterin level and total PRESS score, granulocytes and CRP. In our study we tried to detect the cutoff point of Neopterin to detect pneumonia calculated from ROC curves it was > 13.93 (nmol/L), and the area under the curve of Neopterin was0.866 with sensitivity 92.3% and specificity 77.8%.<br />Conclusion: Neopterin is a useful prognostic marker for pneumonia in Pediatrics. The present study suggests that Neopterin is a promising biomarker for reflecting the severity of Pneumonia.Zagazig University, Faculty of MedicineZagazig University Medical Journal1110-143126120200101Association Between BAFF Gene Polymorphism And Clinical course of Newly Diagnosed Immune Thrombocytopenic Purpura In Children1651734072910.21608/zumj.2019.7944.1043ENEbtihaj AlmukhtarAlhejnyPediatric department, faculty of Medicine, Zagzig UniversityMervat AbdallahHeshampediatric department, faculity of medicine, Zagazig university099776655460009Marwa ZakariaMohamedpediatric department, faculity of medicine, zagazig university0000-0003-3562-7789Amal FawzyAbdelmaguidbiochemistry department, faculity of medicine, zagazig universityJournal Article20190212Background : Primary immune thrombocytopenia is an autoimmune disorder characterized by autoantibody-mediated enhanced platelet destruction. BAFF gene polymorphisms increases BAFF expression and antibody production in ITP patients, which causes platelet destruction and megakaryopoiesis suppression<br />Objective: To study the frequency of BAFF gene polymorphisms in newly diagnosed ITP in children and their association with it's clinical features and course.<br />Methods: A case control study was conducted at Hematology Unit of pediatric department and Medical Biochemistry &Molecular Biology department at Zagazig University Hospital during a period from Nov. 2017 until Nov. 2018.The study Included 40 patients with newly diagnosed ITP (25 males and 15 females) and 20 age and sex-matched healthy children (11males and 9 females) as a control group. All children were subjected to a detailed medical history ,thorough clinical examination and Laboratory investigations including CBC , BM aspiration and B cell activating factor gene polymorphism (-871C/T) detection by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method.<br />Results: There was also a highly significant difference between ITP cases and control regarding distribution of SNP of BAFF gene where CT was founded in 55% of ITP cases versus 25% in control; also, CC was <br />founded in 75% of control versus 20% of cases. There was non-significant difference between different subtypes <br />of BAFF gene polymorphism regarding age, gender, initial bleeding events,CBC, type of treatment and outcome of ITP cases. <br />Conclusion: Significant association of BAFF gene polymorphism with newly diagnosed immune thrombocytopenic purpura in children may indicate its possible role in disease pathogenesis.Zagazig University, Faculty of MedicineZagazig University Medical Journal1110-143126120200101APELIN INDUCED MODULATION OF UTERINE CONTRACTILITY IN ADULT ALBINO RATS AND ITS POSSIBLE MECHANISM/S OF ACTION1741852988310.21608/zumj.2019.11148.1157ENAli KhalilAsalahdepartment of physiology , faculty of medicine , Zagazig universitySherif WagihMansourphysiology department , faculty of medicine zagazig universityKamelia IbrahimAttiadepartment of physiology . faculty of medicine , zagazig univeristySomya El-sayedMahdiPhysiology department faculty of medicine Zagazig universityJournal Article20190329ABSTRACT<br />Background: Apelin is an endogenous ligand for the G protein-coupled receptor APJ , The expression of both apelin and APJ has been detected in a variety of tissues including heart, ,ovary , placenta and uterus. It has a relaxant effect on smooth muscles of the stomach and blood vessels. However , its effect and mechanism of action on the smooth muscle of the uterus are still controversy<br /> <br />Aim of the study : This study was designed to demonstrate the in vitro effects and mechanism of action of apelin on rat uterine reactivity. <br /><br />Material and Methods: 60 adult albino rats (48 females and 12 males). Female rats were randomly divided into non- pregnant, pregnant (day 6 and day 19 ) and 1st day postpartum groups. The effects of apelin (1,10 and 100 nmol /L) on spontaneous reactivity of isolated uterine strips were studied. Also, apelin (100 nmol/L) in the presence of L-NAME , apamin and Glibenclamide was investigated.<br />Results: Apelin exerted a significant dose dependent reduction in frequency and amplitude of spontaneous uterine contraction. This reduction was significantly more potent on day 6 than that in both non pregnant and pregnant rats on day 19. Also , it was significantly and nearly completely abolished in the presence of L-NAME, but it was significantly and partially decreased in the presence of (Apamin) and (Glibenclamide). <br />Conclusion: Apelin has a potent utero-relaxant effect which is greater in early pregnancy compared with late pregnancy. Thus Apelin may be a promising tocolytic drug.Zagazig University, Faculty of MedicineZagazig University Medical Journal1110-143126120200101EFFECT OF EXPOSURE TO ELECTROMAGNETIC RADIATION ON SEX STEROIDS AND SYSTEMIC & LOCAL UTERINE REDOX STATUS DURING EARLY AND LATE PREGNANCY IN RATS1861953203710.21608/zumj.2019.12044.1206ENEmanMahmoudMedical Physiology,faculty of medicine, Zagazig University, Zagazig ,EgyptAkmalDiabMedical physiology, faculty of medicine, Zagazig university, Zagazig, EgyptMohamedIbrahimMedical physiology,faculty of medicine, Zagazig university,Zagazig , EgyptShereenBedeerMedical physiology, faculty of medicine, Zagazig university, Zagazig, EgyptJournal Article20190421Background: pregnant women are at high risk of exposure to environmental EMR because of their higher oxygen consumption and amniotic fluid-induced ROS, which is associated with adverse pregnancy outcomes. <br />Objective: The present study was designed to demonstrate the effects of EMR exposure at 900, 1800, and 2450 MHz on the female sex hormones (estrogen, progesterone) and systemic and local uterine redox status during early and late stages of pregnancy in adult albino rats. <br />Design: The present study was carried out on 88 weaned albino female rats were divided into four equal groups: control group: rats were not exposed to EMR, group2: rats were exposed to 900 MHz EMR, group3: rats were exposed to 1800 MHz EMR (emitted from mobile phones), and group 4: rats were exposed to 2450 MHz EMR (emitted from Wi-Fi). After induction of pregnancy each group was subdivided into two equal groups: early pregnant and late pregnant subgroups. At the end of each experimental period samples were collected for estimation of serum estradiol & progesterone and plasma and local oxidative stress markers.<br />Results: The present study showed that EMR exerted a significant frequency and duration dependent reduction in serum estrogen, progesterone, plasma TAS, vitamin C and uterine GSH which was accompanied by significant increase in plasma and uterine MDA. <br />Conclusion: Exposure to EMR induced progressive reduction in sex steroid hormones, and imbalance in oxidative/antioxidative stress parameters in pregnant rats. These effects were dependent on the frequency and the duration of the EMR