Association of NF-κB1 Gene Polymorphism with Diabetic Kidney Disease Risk in Type 2 Diabetics

Document Type : Original Article


1 Medical Biochemistry and molecular biology department, Faculty of Medicine- Zagazig University, Zagazig city, Egypt

2 Medical biochemistry and molecular biology department, faculty of medicine ,zagazig university

3 Internal Medicine depatment, faculty of medicine, Zagazig University

4 Medical biochemistry and molecular biology department , faculty of medicine, Zagazig university


Background: Diabetic kidney disease (DKD) is the most common cause of end-stage renal failure and a significant contributor to cardiovascular mortality in diabetics. The development of diabetic complications is actively triggered by oxidative stress and low-grade inflammation produced by NF-κB. Objectives: to investigate the relation between Nuclear factor kappa B subunit 1(NF-κB1 )gene polymorphism and DKD in type 2 diabetic patients attending Zagazig University Hospitals. Methods: we included three groups: 1st group was a control group and consisted of 33 apparently healthy individuals, the 2nd group was T2DM without nephropathy, and the 3rd group was T2DM with nephropathy, each of them had the same number of patients (33). NF-κB1 gene polymorphism (rs28362491) was analyzed by restriction fragment length polymorphism reaction. Results: we found that Ins/Ins genotype of NF-κB1 prevalence were significantly higher among T2DM with nephropathy patients than the other two groups. In addition, in T2DM with nephropathy group, Ins/Ins genotype had significantly lower albumin and total protein concentration compared to other 2 genotypes. Conclusion: There was a significant relation between NF-κB1 gene polymorphism and DKD risk in T2DM.
Keywords: Type 2 diabetes mellitus, Diabetic kidney disease, NF-κB1 gene, Polymorphisms, genotypes, nephropathy.


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