Chronic kisspeptin-10 ameliorates osteoporotic changes in orchidectomized male albino rats: involvement of Bone morphogenic protein-2 and Wnt/β-catenin signaling pathways

Document Type : Original Article

Authors

1 Physiology department, Faculty of Medicine, Zagazig University, Egypt

2 Pathology department faculty of Medicine, Zagazig University

3 Physiology department faculty of medicine Zagazig university Zagazig Sharkia

Abstract

Osteoporosis is a serious worldwide skeletal illness manifested by diminished bone density with increased susceptibility to fractures and increases with aging. Kisspeptin is a hypothalamic neuropeptide that regulates the gonadal hormones and was suggested to have a positive effect on bone metabolism. Material & Methods: 30 male rats were divided equally in to three groups: Sham, orcidectomized (ORX), and kisspeptin-10 (kiss)-treated groups. Blood, urine, and femoral bone samples were taken for biochemical, histopathological, and gene studies. Results: ORX rats showed increased serum levels of alkaline phosphatase (ALP), osteocalcin, RANKL, Osteopetrogenin (OPG), RANKL/ OPG ratio, tumor necrosis factor alpha (TNF-α) and interleukin 6 (IL-6(, while decreased serum OPG level and bone minerals associated with disruption of bone architecture compared with Sham group. Chronic treatment with kisspeptin-10 significantly enhanced bone architecture, increased bone minerals, improved RANKL/OPG ratio, up-regulated mRNA expression levels of OPG, bone morphogenic protein 2 (BMP-2), Wnt3a, and β-catenin as compared to untreated ORX rats. Conclusion: Chronic treatment with kisspeptin-10 enhances osteogenesis and reduces bone resorption in ORX rats via modulating RANKL/OPG ratio, BMP-2, and Wnt/β-catenin signaling pathways. So, the present study recommends kisspeptin-10 as a promising novel therapeutic agent for age-related osteoporosis in men.

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Zagazig University Medical Journal
Volume 30, Issue 8.1
November 2024
Pages 3992-4002

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