Document Type : Original Article
Authors
1
Internal Medicine Department, Faculty of Medicine, Zagazig University
2
Head of Hematology Unit, Internal Medicine Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt
3
Medical Biochemistry Department, Faculty of Medicine, Zagazig University, Egypt
Abstract
Background: The gene expression of several cytokines can affect the clinical outcome of type 2 diabetes mellitus (T2DM) with chronic kidney disease (CKD). This study aim was to assess the effect of IL-4 and IL-6 gene expression in T2DM cases with CKD.
Patients and methods: This case control study was performed on 78 subjects; they were allocated into three groups; Group (1) normal individuals, Group (2) T2DM cases without CKD, and Group (3) T2DM cases with CKD having 26 subjects each. IL6 and IL-4 gene expression of all cases were assessed by real-time PCR.
Results: A notable positive association was detected between IL-6 and body mass index, duration of diabetes, blood pressure, blood glucose levels, HbA1c, kidney function measures like albumin/creatinine ratio, creatinine, urea, and estimated GFR, lipid levels like total cholesterol, LDL-C and triglycerides (p<0.001) while there was a strong negative relationship between IL-6 and serum albumin and IL-4 levels (p<0.001). However, There was a strong negative correlations between interleukin-4 (IL-4) levels and body mass index, duration of diabetes, blood pressure, glucose levels, HbA1c, kidney dysfunction markers, total cholesterol, LDL-C, triglycerides (p<0.001). On the other hand, a positive correlation between interleukin-4 (IL-4) levels and serum albumin, estimated GFR, and HDL-C was found (p<0.001).
Conclusion: IL6 and IL4 expression could contribute to CKD progression in cases with T2DM. Screening genes that influence the risk of CKD and T2DM could eventually allow the identification of individuals at risk, further multicenter studies are needed to confirm these findings.
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