Document Type : Original Article
Authors
1
Department of physiology, Faculty of medicine, Zagazig University, Egypt
2
Physiology department, Faculty of medicine,Zagazig university,Zagazig, Egypt
3
Department of Human Anatomy and Embryology, Faculty of Medicine, Zagazig University, Egypt
4
Clinical pharmacology department faculty of medicine zagazig university
5
Physiology department, Faculty of Medicine, Zagazig University, Egypt.
Abstract
Background:In postmenopausal osteoporosis, hormonal alteration leads to bone turnover and muscle weakness. Activin typeIIB receptors were revealed to increase bone & muscle mass. This experiment was designed to explore ActRIIB-Fc-ligands administration alone or with swimming-exercise might restore musculoskeletal system homeostasis followed negative effects of estrogen deficiency in ovariectomized rat model.
Methods:Fifty adult female albino rats were divided into 5groups: Group I (control sham rats) were subjected to i.p. PBS. In the other groups rats were exposed to ovariectomy procedures. Group II, III, IV rats were exposed to i.p. PBS, exercise-training-protocol, i.p. ActRIIB-Fc (5mg/kg/week) 7weeks respectively, while groupV rats were exposed to exercise-training-protocol and i.p ActRIIB-Fc. Finally, body weights & muscle strength tests were examined for all animals. After scarification, uterine weights and muscle mass (quadriceps femoris & gastrocnemius) measurements were obtained. Serum biochemical and histopathological parameters were examined; also, RNA was extracted from their right femora to perform genes expression.
Results:Exercise-training and ActRIIB-Fc were capable to reverse changes of muscle strength and bone mass measurements of OVX rats. Significant elevation in osteocalcin & P1NP and significant reduction in CTX, TOS, TAC, IL-1β, IL-6, and CK values, moreover, down-regulation of RANKL and up-regulation of OPG genes expression were detected. These significant values and histomorphometric osteoporotic variations were more pronounced in groupV.
Conclusion:Addition of ActRIIB-Fc ligand to exercise-training-protocol acts as a musculoskeletal-enhancing agent in OVX rats. This result was proved by reduction of osteoclast function and elevation of some osteoblast genes expression;therefore, it could be a respectable choice in musculoskeletal disorders management.
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