Assessment of Interleukin-22 mRNA as a Diagnostic Marker for Polycystic Ovary Syndrome -Associated Metabolic Dysfunction

Rashad, Nearmeen; Saraya, Yaser Samir; Abdel Aziz, Eman; Gobran, Amira Mokhtar; Atef, Rehab; Abd El-Fatah, Azza Hassan; Mousa, Mayada

doi:10.21608/zumj.2024.305018.3481

Assessment of Interleukin-22 mRNA as a Diagnostic Marker for Polycystic Ovary Syndrome -Associated Metabolic Dysfunction

Document Type : Original Article

Authors

¹ Diabetes and Endocrine Unit -Internal Medicin Departement -Zagazig University

² Gynecology and Obstetrics, Faculty of Medicine, Zagazig University

³ clinical pathology department, faculty of medicine, Zagazig university

⁴ phsiology department . faculty of medicine zagazig university

⁵ Medical Biochemistry Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt Biochemistry Department, College of Medicine, Taif University, Taif, Saudi Arabia

⁶ Lecturer of Internal Medicine, Faculty of Medicine, Zagazig University

⁷ zagazig university

10.21608/zumj.2024.305018.3481

Abstract

Abstract

Background: Metabolic abnormalities and immune dysregulations are the most common risk factors for polycystic ovary syndrome (PCOS). Interleukin-22 (IL-22) is implicated in the pathogenesis of the immune-inflammatory system in the context of metabolic dysfunction. In the current research, we aimed to investigate IL-22 mRNA in PCOS and explore its correlations with metabolic syndrome (MetS) and reproductive features of PCOS.

Methods: we conducted our research on 60 women as control groups, and 70 patients with PCOS (30 patients without MetS and 40 patients with MetS). We investigated serum IL-22 by ELISA. At the same time, the IL-22 mRNA level was analyzed by RT-qPCR.

Results: Among 70 women with PCOS, 40 (57.1%) had metabolic syndrome. women with PCOS had significantly lower serum and mRNA Expression Levels of IL-22 compared to control. Even more importantly, among PCOS patients, women with MetS had significantly lower serum and mRNA Expression levels of IL-22 compared to women without MetS. serum and mRNA Expression levels of IL-22 were negatively associated with MetS components and inflammatory markers as well as reproductive features of PCOS. The AUC of serum and mRNA levels of IL-22 in the prediction of MetS among PCOS women had a sensitivity of 90%, and 77.5%, respectively, and specificity of 66%, 87.3% and respectively.

Conclusion: PCOS patients had significantly serum and mRNA Expression levels of IL-22 than controls. the lowered IL-22 serum and mRNA levels could be used as diagnostic markers of PCOS patients more specifically women with MetS.

Keywords