Document Type : Original Article
Authors
1
Diabetes and Endocrine Unit -Internal Medicin Departement -Zagazig University
2
internal medicine department -zagazig university
3
Department of Medical Oncology, Faculty of Medicine, Zagazig University, Egypt
4
Topical Medicine Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt
5
Clinical Pathology Department-faculty of medicine, zagazig university
6
Clinical Pathology Department, Faculty of Medicine, Zagazig University, Egypt
7
Pharmacology department-zagazig university
8
Medical Biochemistry Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt Biochemistry Department, College of Medicine, Taif University, Taif, Saudi Arabia
9
Hepatology and Gastroenterology and Endoscopy Unit-Zagazig University
Abstract
Background: Diffuse large B-cell lymphoma (DLBCL) has heterogeneity in its behavior, and pathological and molecular identification. Hematopoiesis is regulated by microRNAs thus its aberrant expression predisposes to myeloid and lymphoid neoplasms the current research aimed to explore serum miR-26a-5p and miR-199b-5p in patients with DLBCL and to evaluate its correlation with clinicopathological features and treatment protocols of NHLs among Egyptian patients with Hepatitis C virus (HCV)Infection.
Methods: We enrolled 60 participants; 30 patients with DLBCL and 30 healthy persons as the control group. all subjects were screened for the presence of HCV-RNA in both plasma and PBMCs. miR-26a-5p and miR-199b-5p levels were determined by RT-PCR.
Results: Serum miR-26a-5p and miR-199b-5p level values were downregulated in DLBCL patients with HCV and OCI compared to non-HCV and controls. There were significantly higher values of both miR-26a-5p and miR-199b-5p levels in patients with complete response(n=15,50%) compared to partial response (n= 5,16.7%) and primary refractory (n=10,33.3%), p˂0.001. Noteworthy, there were statistically significant negative correlations between miR-26a-5p, miR-199b-5p, LDH, and total bilirubin. Linear regression tests revealed that among examined associated variables, only LDH was the independent variable associated with miR-26a-5p and miR-199b-5p, P ˂0.001*
Conclusions:
Serum miR-26a-5p and miR-199b-5p levels were downregulated in DLBCL patients, especially with HCV infection. Thus, these epigenetic markers could provide a new era of precision medicine to better refine diagnosis, prognostication, and rational treatment
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