• Home
  • Browse
    • Current Issue
    • By Issue
    • By Author
    • By Subject
    • Author Index
    • Keyword Index
  • Journal Info
    • About Journal
    • Aims and Scope
    • Editorial Board
    • Publication Ethics
    • Indexing and Abstracting
    • Peer Review Process
  • Guide for Authors
  • Submit Manuscript
  • Reviewers
  • Contact Us
 
  • Login
  • Register
Home Articles List Article Information
  • Save Records
  • |
  • Printable Version
  • |
  • Recommend
  • |
  • How to cite Export to
    RIS EndNote BibTeX APA MLA Harvard Vancouver
  • |
  • Share Share
    CiteULike Mendeley Facebook Google LinkedIn Twitter
Zagazig University Medical Journal
arrow Articles in Press
arrow Current Issue
Journal Archive
Volume Volume 28 (2022)
Volume Volume 27 (2021)
Volume Volume 26 (2020)
Volume Volume 25 (2019)
Volume Volume 24 (2018)
Volume Volume 23 (2017)
Volume Volume 22 (2016)
Volume Volume 21 (2015)
Volume Volume 20 (2014)
Issue Issue 6
Issue Issue 5
Issue Issue 4
Issue Issue 3
Issue Issue 2
Issue Issue 1
Volume Volume 19 (2013)
Y, E., K, E., M, G., M, A. (2017). EXPRESSION OF GLYPICAN-3(GPC-3) AND 8-HYDROXYDEOXYGUANOSINE (8-OHDG) IN CHRONIC HEPATITIS C AND RELATIONSHIP WITH HEPATOCELLULAR CARCINOMA. Zagazig University Medical Journal, 20(6), 1-12. doi: 10.21608/zumj.2017.4441
El-Alfy Y; El-Kashishy K; George M; Abdel-Wahab M. "EXPRESSION OF GLYPICAN-3(GPC-3) AND 8-HYDROXYDEOXYGUANOSINE (8-OHDG) IN CHRONIC HEPATITIS C AND RELATIONSHIP WITH HEPATOCELLULAR CARCINOMA". Zagazig University Medical Journal, 20, 6, 2017, 1-12. doi: 10.21608/zumj.2017.4441
Y, E., K, E., M, G., M, A. (2017). 'EXPRESSION OF GLYPICAN-3(GPC-3) AND 8-HYDROXYDEOXYGUANOSINE (8-OHDG) IN CHRONIC HEPATITIS C AND RELATIONSHIP WITH HEPATOCELLULAR CARCINOMA', Zagazig University Medical Journal, 20(6), pp. 1-12. doi: 10.21608/zumj.2017.4441
Y, E., K, E., M, G., M, A. EXPRESSION OF GLYPICAN-3(GPC-3) AND 8-HYDROXYDEOXYGUANOSINE (8-OHDG) IN CHRONIC HEPATITIS C AND RELATIONSHIP WITH HEPATOCELLULAR CARCINOMA. Zagazig University Medical Journal, 2017; 20(6): 1-12. doi: 10.21608/zumj.2017.4441

EXPRESSION OF GLYPICAN-3(GPC-3) AND 8-HYDROXYDEOXYGUANOSINE (8-OHDG) IN CHRONIC HEPATITIS C AND RELATIONSHIP WITH HEPATOCELLULAR CARCINOMA

Article 1, Volume 20, Issue 6, November 2017, Page 1-12  XML PDF (1.89 MB)
Document Type: Original Article
DOI: 10.21608/zumj.2017.4441
Authors
El-Alfy Y; El-Kashishy K; George M; Abdel-Wahab M
Pathology Department, Faculty of Medicine, Zagazig University
Abstract
Background: In Egypt, hepatocellular carcinoma (HCC) is the second most common cancer in men and the 6th most common cancer in women. Egypt has the highest prevalence of HCV in the world and the prevalence of HCC is increasing in the last years. Glypican-3 (GPC-3), a member of heparin sulfate proteoglycans, plays a role in cell growth, differentiation, and migration. 8-Hydroxydeoxyguanosine (8-OHdG) is an oxidatively modified promutagenic DNA that is produced by oxygen radicals and is recognized as a useful marker in estimating DNA damage induced by oxidative stress.
The aim: This study was conducted to evaluate glypican-3 (GPC-3) and 8-hydroxydeoxyguanosine (8-OHdG) expression in chronic hepatitis C and hepatocellular carcinoma as predictive markers for hepatocellular carcinoma.
Methods: 75 cases (50 cases of chronic hepatitis C and 25 of hepatocellular carcinoma) were examined immunohistochemically using antibodies against Glypican-3 (GPC-3) and 8-Hydroxydeoxyguanosine (8-OHdG).
Results: Glypican-3 expression was observed in 18 % of cases of chronic hepatitis C, all positive cases were in the high grade, while none of low grade chronic hepatitis cases showed glypican-3 positivity. Glypican-3 expression was observed in 88% of cases of HCC. High 8-OHdG index was observed in 72.4% of cases of low grade chronic hepatitis and 95.2% of cases of high grade chronic hepatitis, while low index was observed in 27.6% of low grade chronic hepatitis and in 4.8% of cases of high grade chronic hepatitis. There was a statistically significant difference in 8-OHdG expression between low and high grade chronic hepatitis (P=0.05). There was no statistically significant difference in glypican-3 and 8-OHdG expression between different grades of HCC.
Conclusion: Glypican-3 was expressed in 18% of cases of chronic hepatitis C, 35.1% of cirrhosis and 36.4% of cirrhosis adjacent to hepatocellular carcinoma. Our results suggest that GPC-3 may be considered as an early marker of liver carcinogenesis. 8-OHdG was observed in all cases of chronic hepatitis C, with high labeling index in 82% of cases and low index in 18% of cases. Our study showed high labeling index of 8-OHdG in 52% of cases of HCC. These results indicate that oxidative DNA damage is common in livers with chronic injury suggesting a possible link between chronic inflammation and hepatocarcinogenesis.
Keywords
Glypican-3(GPC-3); 8-Hydroxydeoxyguanosine (8-OHdG); Chronic hepatitis C; Hepatocellular carcinoma; Immunohistochemistry
Statistics
Article View: 758
PDF Download: 456
Home | Glossary | News | Aims and Scope | Sitemap
Top Top

Journal Management System. Designed by NotionWave.