Relation of Vaspin rs2236242 Gene Polymorphism to Type-2 Diabetic Patients with Coronary Artery Disease

Hassan, Eman Kamel; Mohamad, Samy Hassan; Hassan, Mohamad Mohamad Mohamad; Mosaad, Hala

doi:10.21608/zumj.2025.403720.4053

Relation of Vaspin rs2236242 Gene Polymorphism to Type-2 Diabetic Patients with Coronary Artery Disease

Document Type : Original Article

Authors

¹ Demonstrator of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Zagazig University

² Professor of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Zagazig University

³ Professor of Internal Medicine, Faculty of Medicine, Zagazig University

10.21608/zumj.2025.403720.4053

Abstract

Background: Type 2 diabetes mellitus (T2DM) as well as coronary artery disease (CAD) are highly prevalent and often coexist, especially among high-risk populations like Egypt. Recent research suggested that genetic variants, involving the vaspin (SERPINA12) gene, could play a role in the development of T2DM and the related vascular complications. This work aimed to assess the association of the vaspin rs2236242 gene polymorphism with T2DM and the presence of CAD in Egyptian patients, and to assess its potential as a genetic risk factor.

Methods: We carried out this case-control study on 90 participants divided into three groups: healthy controls (n=30), T2DM patients without CAD (n=30), and T2DM patients with CAD (n=30). Clinical, biochemical, and genetic data were collected. Genotyping of vaspin rs2236242 was performed using TARMS PCR. Gel electrophoresis was utilized for analysis of the PCR products following the amplification of the Vaspin rs2236242 polymorphism.

Results: The TA genotype frequency of vaspin rs2236242 was significantly higher in T2DM (56.7%) and T2DM with CAD (83.3%) versus controls (23.3%) (P < 0.001), and A allele frequency also increased across groups (P < 0.001). No AA genotype was detected. TA carriers had significantly higher fasting glucose, HbA1c, and blood pressure, especially among the CAD group (P < 0.01).

Conclusion: This study suggests that the vaspin rs2236242 gene polymorphism particularly the TA genotype may play a significant role in the development of type 2 diabetes mellitus, especially in patients who also have coronary artery disease.

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