Document Type : Original Article
Authors
1
Physiology Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt
2
Physiology department, Faculty of Medicine, Zagazig University, Zagazig, Egypt
3
physiology department, Faculty of Medicine, Zagazig University
Abstract
Background: Cardiovascular diseases such as acute myocardial infarction(AMI) are major causes of morbidity and mortality all over the world,where obesity is a major risk factor for such diseases. Adropin,which is a peptide synthesized in the heart,is suggested to be released into the bloodstream after ischaemic cardiac injury.
Aim: To investigate the effect of the ISO-induced AMI in normal and obese rats on serum adropin level and to define any potential association between its level and other cardiac enzyme markers,oxidative stress markers,proinflammatory and metabolic parameters.
Material and methods: Eighty seven adult male albino rats were used.Seventeen animals died and the remaining animals(70) were divided into 5main groups:control group and four ISO-induced AMI groups where blood samples and hearts were taken at 1h.,2h.,6h. and 24 h.post AMI.Each group is subdivided into lean and obese subgroups.In all groups, serum adropin,cTn-I,CK-MB,CRP,TNF-α,oxidative stress markers(MDA,SOD,GSH and catalase),lipid profile,insulin and glucose levels were measured.HOMA-IR was calculated.ECG and blood pressure were recorded. Histopathological examination of the heart was done.
Results: Our results showed that serum adropin levels were increased after AMI together with cTn-I,CK-MB,CRP,TNF-α,MDA,TC,TGs and LDL-C. Adropin showed positive correlation with abovementioned parameters in all studied groups. SOD,GSH,catalase and HDL-C were decreased after AMI with negative correlation with adropin in all groups. Histopathological examination revealed edema, inflammatory cell infiltration,congestion,haemorrhage and necrosis in ISO-induced AMI.
Conclusions: Serum adropin can be used as an alternative biomarker to other cardiac enzyme markers(cTn-I,CK-MB)for early diagnosis of AMI especially in cases of obesity.
Key words:adropin,acute myocardial infarction,oxidative stress markers.
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