FMS-like tyrosine kinase 3 (Internal Tandem Duplication) gene mutation in Acute Myeloid Leukemia patients and its association with CD34

Mohamed Elprince, Reem Alaa Eldeen; Ahmad, Ebtesam Ebrahim; Abdel-motty, Alaa; Haggag, Rasha

doi:10.21608/zumj.2020.23639.1726

FMS-like tyrosine kinase 3 (Internal Tandem Duplication) gene mutation in Acute Myeloid Leukemia patients and its association with CD34

Document Type : Original Article

Authors

¹ clinical pathology, faculty of medicine, zagazig university, el sharkeya, egypt

² clinical pathology, faculty of medicine ZAG.university

³ Faculty of Medicine, Zagazig University, Department of Clinical Pathology, Sharkia Egypt

⁴ medical oncology department, faculty of medicine, zagazig university, sharkia, egypt

10.21608/zumj.2020.23639.1726

Abstract

Background: Acute myeloid leukemia (AML) is a heterogeneous disorder characterized by clonal expansion of myeloid progenitors in the bone marrow and peripheral blood Objectives: This study aimed to assess the role of FLT3 (ITD) gene mutation in AML patients and to find out the impact of the CD34 expression in these cases. Subjects : 30 subjects of adult patients with newly diagnosed AML were included in this study. Results: Patients were classified into favorable (6.6%), intermediate (80%) and unfavorable (13.3%) according to cytogenetic abnormalities. Mean value of CD34% (± SD) among cases was 45.53±25.32 with median 35.85 and range (5-91), While it was 42.52±29.9 with median 30.85 and range (5-91) among FLT3-ITD positive cases and 44.21±22.67 with median 39.3and range (16-88) among FLT3-ITD negative cases with no significant statistical relation between FLT3-ITD gene and CD34, P (0.672). CD34 positivity among FLT3-ITD positive cases showed 9 cases (75%) CD34% positive and 3 cases (25%) CD34% negative while among FLT3-ITD negative cases there were 15 cases (83.3%) CD34% positive and 3 cases (16.7%) CD34% negative, with no significant statistical relation. As regard the outcome, among FLT3-ITD positive cases 5 cases (41.7%) underwent relapse and 7 cases (58.3%) died. On the other hand among FLT3-ITD negative cases one case (5.6%) underwent relapse and 11 cases (61.1%) died, There is significant elevation in the relapsed cases among the FLT3-ITD positive cases (P = 0.025), while there is no significant relation between FLT3-ITD gene and death with (P = 0.879).

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