Cytokine Storm in COVID-19 Patients: Association between cytokines and disease severity

Sarhan, Walaa M.; Shalaby, Sally M; Zidan, Nahla; Elhawary, Abeer; Ismail, Nagwan; Makani, Vishruti; Abd Elnour, Hanim Magdy

doi:10.21608/zumj.2021.76794.2237

Cytokine Storm in COVID-19 Patients: Association between cytokines and disease severity

Document Type : Original Article

Authors

¹ Medical Biochemistry &amp; Molecular Biology department, Faculty of Medicine, Zagazig University

² Medical Biochemistry Department-Faculty of Medicine- Zagazig University-Egypt

³ Clinical Pathology Department, Faculty of Medicine, Zagazig University

⁴ Chest Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt

⁵ Chest Department, Faculty of Medicine, Zagazig University

⁶ Process Development Scientist, KBI Biopharma, Durham, NC, US

⁷ medical biochmestry and molecular biology department, faculty of medicine, Zagazig univerisity, Zagazig, Egypt

10.21608/zumj.2021.76794.2237

Abstract

Objectives: To determine the cytokine storm profile in patients with COVID-19 infection in relation to the disease severity.
Methods: Serum levels of TNF-α, IFN-γ, IL-6 and IL-1β were measured by ELISA method and IL-1RII, TNFR1 and TNFR2 gene expression in peripheral blood mononuclear cells (PBMCs) were done using real time polymerase chain reaction (PCR) method in a total of 203 COVID-19 patients. The 1st group included 52 COVID-19 severe cases, the 2nd comprised 104 moderate cases and the 47 mild cases lied in the 3rd group.
Results: There was a significant association between degree of COVID-19 severity and IL6, TNF-α & IFN-γ serum levels (p < /p>

Keywords